Journal article
Diagnosing malaria infected cells at the single cell level using focal plane array Fourier transform infrared imaging spectroscopy
BR Wood, KR Bambery, MWA Dixon, L Tilley, MJ Nasse, E Mattson, CJ Hirschmugl
Analyst | ROYAL SOC CHEMISTRY | Published : 2014
DOI: 10.1039/c4an00989d
Abstract
New methods are needed to rapidly identify malaria parasites in blood smears. The coupling of a Focal Plane Array (FPA) infrared microscope system to a synchrotron light source at IRENI enables rapid molecular imaging at high spatial resolution. The technique, in combination with hyper-spectral processing, enables imaging and diagnosis of early stage malaria parasites at the single cell level in a blood smear. The method relies on the detection of distinct lipid signatures associated with the different stages of the malaria parasite and utilises resonant Mie extended multiplicative scatter correction to pre-process the spectra followed by full bandwidth image deconvolution to resolve the sin..
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Funding Acknowledgements
We acknowledge travel funding provided by the International Synchrotron Access Program (ISAP) managed by the Australian Synchrotron and funded by the Australian Government. BRW is supported by an Australian Research Council Future Fellowship (FT120100926). We acknowledge funding from the Australian Research Council and the National Health & Medical Research Council. We also acknowledge the Multi-modal Australian Science Imaging and Visualisation Environment (MASSIVE) (http://www.massive.org.au). This work has been done with support from a National Science Foundation (NSF) Major Research Instrumentation grant (DMR-0619759) and also NSF Chemistry: CHE-1112234. This research is based upon work performed at the Synchrotron Radiation Center. The SRC is funded by the University of Wisconsin-Madison and University of Wisconsin-Milwaukee and through user fees.